Watermelon citrullus lanatus citrullus lanatus subject category. Manchaaquosa do melao causada por acidovorax avenae subsp. Factors influencing the detection of acidovorax citrulli. Acidovorax citrulli is the causal agent of bacterial fruit blotch bfb, a sporadic but devastating disease of cucurbits worldwide schaad et al. Differential expression of in vivo and in vitro protein. Acidovorax citrulli schaad et al, 2008 is a nonsporulating bacterium, which is derived from saprophytic relatives within the genus through acquisition of plant virulence genes. Walcott rr, langston db jr, sanders, fh jr, gitaitis rd, flanders tj, 2000 natural outbreak of a bacterial fruit rot of cantaloupe in georgia caused by acidovorax avenae subsp. The relationship between initial acidovorax avenae subsp. Usda aphis ppq permit 526, plant pathogens permit must be obtained and a copy of the permit must be sent to atcc in advance of shipment. Abstract to assess the diversity of acidovorax avenae subsp. The pure cultures were tested for pathogenicity by infiltrating 0. First report on the occurrence of bacterial stripe organism acidovorax avenae subsp. Because these bacteria are closely related their proper identification requires expensive, timeconsuming. Reclassification of subspecies of acidovorax avenae as a.
Evaluation of seed treatments for the eradication of. Acidovorax citrulli strain aac001 acidovorax avenae subsp. The invention also relates to the use of primers in polymerase chain reaction pcr assays for the. Holeva mc, karafla cd, glynos pe, alivizatos as 2010 acidovorax avenae subsp. Selective detection of viable seedborne acidovorax. Although limited resistance has been found in a small number of plant introductions pis in watermelon citrullus spp. Screening of plant epiphytic yeasts for biocontrol of. Selective detection of viable seedborne acidovorax citrulli by real.
Assis s m p, mariano r l r, silvahanlin d m w, duarte v, 1999. Transfer of several phytopathogenic pseudomonas species to acidovorax as acidovorax avenae subsp. Disease names include bacterial fruit blotch of watermelon and fruit blotch. Vertical bars represent the standard errors of means n 3. Acidovorax citrulli psdmacoverview eppo global database. A single unique band of approximately 360 bp was amplified for all strains tested.
Isolates are recovered from symptomatic seedlings and identified, then confirmed with a pathogenicity assay. Plant pathogenic bacteria are not adapted to survive as free cells in the. Us20030190658a1 diagnostics for the detection of acidovorax. Pdf an immunomagnetic separation and polymerase chain reaction. Use of pcr for rapid identification of acidovorax avenae and. The phytopathogenic bacteria acidovorax avenae subsp. The bacterium acidovorax avenae causes disease in a wide range of economically important monocotyledonous and dicotyledonous plants, including corn, rice, watermelon, anthurium, and orchids. Walcott rr, gitaitis rd, castro ac 2003 role of blossoms in watermelon seed infestation by acidovorax avenae subsp. Control of the disease is based primarily on use of pathogen free seed. These permits may be required for shipping this product. To determine the effectiveness of several treatments to eradicate it from seeds, healthy triploid watermelon seed lots were spiked with naturally infected watermelon and melon seeds harvested from three separate diseased fruits.
Other names i atcc 29625 acidivorax citrulli acidovorax avenae subsp. Rnase free water and checked for presence of pcr inhibitors. The pathogen is also known as acidovorax avenae subsp. Genetic diversity analysis of acidovorax citrulli in china. Investigating intraspecific variation of acidovorax avenae. Inoculum of yeasts was from cultures in potato dextrose broth and aac from cultures in kings medium b. Kochs postulates were completed with 8dayold glasshousegrown cucumber cv. Cells were then washed using sterile distilled water and the suspension adjusted to 10 8 cfu ml.
The species acidovorax avenae contains bacteria pathogenic to several crops. Oct 14, 2016 first report of acidovorax avenae subsp. Bacterial fruit blotch bfb caused by the bacterium acidovorax avenae subsp. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. Occurrence, isolation, and identification of acidovorax citrulli from. Target acidovorax citrulli detection primers patented, us patent 6,146,834 54f aacf3 cct cca cca acc aat acg ct 54r aacr2 tcg tca tta ctg aat ttc aac a. First report of a fruit rot of pumpkin caused by acidovorax avenae subsp.
The success of acidovorax citrulli detection by seed health testing of commercial. This was performed to verify that the pis were free of aac or. A lamp primer set was designed for specific amplification of the hrpghrpx gene spacer region in a. August 2018 principle detection of infectious acidovorax citrulli bacteria on cucurbits seedlings by growing out seeds under environmental conditions highly conducive to producing disease symptoms. A new distribution map is provided for acidovorax avenae subsp. Strains of the group i lineage of acidovorax citrulli. The disease was first detected in florida in and was subsequently detected in south. Natural outbreak of a bacterial fruit rot of cantaloupe in georgia caused by acidovorax avenae subsp.
Seedborne inoculum is the most important source of primary inoculum for bfb development in cucurbit seed and fruit production fields as well as in. The disease was first detected in florida in 1989 and. Aug 01, 2009 a histogram showing inhibition of growth of acidovorax avenae subsp. Bacterial fruit blotch of watermelon acidovorax avenae subsp. Polyphasic analysis of acidovorax citrulli strains from. Aug 01, 2019 acidovorax citrulli zju bproteobacteria genome assembly acidovorax avenae subsp. The rcsb pdb also provides a variety of tools and resources.
Six representative strains were identified as acidovorax avenae subsp. Hopkins dl, cucuzza jd, watterson jc 1996 wet seed treatments for the control of bacterial fruit blotch of watermelon. Method for the detection of acidovorax citrulli in seed of. Bacterial fruit blotch caused by acidovorax avenae subsp. Bacterial fruit blotch bfb, caused by acidovorax avenae subsp.
Acidovorax citrulli strain aac001 acidovorax avenae. Two pairs of specific primer sets bxl1bxr5 and bxl1bxsr2 derived from box short repeated. A new distribution map is provided for acidovorax avenae acidovorax avenae subject category. Avoid introduction of the bacterium into production fields by planting diseasefree seed from reputable seed producers. Bacterial fruit blotch is caused by acidovorax avenae subsp. The disease was first detected in florida in 1989 and the disease was first detected in florida in 1989 and was subsequently detected in south carolina, north carolina, maryland, delaware, and indiana as the growing season. Strain designations c42 ccug 1739, icmp 7500, lmg 5376. As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. Immunocapture pcr method for detecting acidovorax avenae. The reaction of individual pis to bfb evaluated in the spray tests was. On the basis of carbon source utilization, dnafingerprinting profiles, wholecell fattyacid composition utilization and pathogenicity assays, a. Acidovorax citrulli type strain ccug 17393, cip 106436. This article is brought to you for free and open access by the plant pathology department at. Cucurbit bacterial fruit blotch caused by acidovorax avenue subsp.
Watermelon citrullus lanatus, melon cucumis melo and other cucurbits. Pm 7127 1 acidovorax citrulli 2016 eppo bulletin wiley. Genotypic and phenotypic relatedness among strains of phytopathogenic a. An immunocapture polymerase chain reaction icpcr method for detection of acidovorax avenae subsp. Ihsin sung, yiru lai, chienjui huang, first report of acidovorax avenae subsp. Us20050196805a1 diagnostics for the detection of acidovorax. This pathotype strain is also available as cfbp 4459 or lmg 5376. Acidovorax avenue citrulli citrullus lanatus thunb. Pseudomonas pseudoalcaligenes citrulli pseudomonas avenae subsp. Tn5 transposon mutagenesis in acidovorax citrulli for. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Selective detection of viable seedborne acidovorax citrulli. In particular, the present invention relates to a novel protein that is specific for a. A slowgrowing, white, gramnegative, nonfluorescent bacterium was consistently isolated. The disease was first reported in 1965 in seedlings of several usda plant introduction pi lines in georgia.
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